C4/Factor B-Dpl

Product Description

C4/FB-Dpl is normal human serum in which C4 and factor B (fB) have been removed by immunoaffinity chromatography. The product is tested for the absence of C4and factor B activity by testing classical and alternative pathway function and for the absence of C4 and factor B proteins by double immunodiffusion. The C4/FB-Dpl is certified to exhibit less than 5% classical pathway and alternative pathway activities. After reconstitution with purified factor B (0.2 mg/mL), C4/FB-Dpl is certified to possess a functional alternative pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). Similarly, a functional classical pathway can be reconstituted by addition of purified C4 (0.4 mg/mL) indicating that all other complement components necessary for classical and alternative pathway activation are present. The absence of C4 also prevents complement activation by the lectin pathway, but the function of this pathway is not tested.

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