Normal rat serum is tested for complement activity and certified to possess functional classical and alternative pathways of activation. NRS was prepared from male mixed breed rats. Each sample of blood was collected without anticoagulants and after coagulation the liquid portion was separated by centrifugation. Serum was filtered through a 0.22 µm filter, aliqoted and frozen at -80oC.
All testing for complement activity was performed on the subsequently thawed samples to guarantee that the functional activity reported is what customers will receive when the samples are thawed. Complement activity is stable for several years if NRS is stored at -70oC or below continuously. Rat complement is not as stable as human complement in normal human serum and a significant degradation of activity may be observed if the NRS serum is allowed to remain thawed for more than a few hours even if it is kept on ice. In addition, a significant loss of activity may occur following additional freeze thaw cycles.
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