Normal human serum depleted of complement C1q protein by immunoaffinity chromatography. The product is tested for the absence of C1q by functional assays for classical pathway activity and for C1q protein by double immunodiffusion. C1q-Dpl is certified to possess a functional alternative pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). A functional classical pathway can be reconstituted by addition of purified C1q protein (70 µg/mL) indicating that all other complement components necessary for classical pathway activation are present and active. The absence of C1q does not prevent complement activation by the lectin and alternative pathways on appropriate surfaces.
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