GVBo is a basic buffer that can be used to make other traditional buffers used for complement assays. Ca++ and Mg++ may be added to GVBo to make GVB++ for classical pathway and lectin pathway assays. MgEGTA may be added to GVBo for alternative pathway assays. EDTA may be added to GVBo to prepare GVBE to inhibit complement activation. GVBo is also used for dilution of serum and other assay components in many complement assays especially in alternative pathway assays (Morgan, B.P. (2000; Dodds, A.W. and Sim, R.B. (1997)).
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